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. 2020 Nov 27;11:596908. doi: 10.3389/fimmu.2020.596908

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Copyright © 2020 Liu, Pan, Gu, Cao, Liu, Zhang, Xiao, Wang, Guo, Ju and Deng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Affinity assessment and biophysical characterization of anti-IgE antibody candidates after affinity maturation. (A) Affinity analysis of antibody candidates by SPR. (B) Expression level of anti-IgE antibody candidates compared with L0H0. (C) The pI values of antibody candidates by iCIEF. (D–F) Charge variants analysis of antibody candidates by iCIEF. (G) Charge variants profiles of antibody candidates by iCIEF. L0H0, an in-house generated omalizumab biosimilar antibody, was used as control. AB1904Am3, AB1904Am6 and AB1904Am10 were chosen as candidates, and YTE mutation in Fc fragment was introduced into them to yield AB1904Am13, AB1904Am16 and AB1904Am15, respectively, for the following stability study.