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. 2020 Nov 27;11:561687. doi: 10.3389/fgene.2020.561687

FIGURE 5.

FIGURE 5

Downregulated BER-associated protein levels in normal and XP-C fibroblasts, post-UVB-irradiation. Protein level was investigated in XP-C vs. control fibroblasts 4 h post-UVB-irradiation (0.05 J/cm2). Total protein was extracted followed by western blot to evaluate protein expression. Values shown are the mean ± SEM from three independent experiments, n = 3. Ratio of IR/NIR was used in analysis after normalization by the total protein. Panel (A) shows the significant downregulation of normalized IR/NIR OGG1 protein expression in XP-C1, XP-C2, and XP-C3 compared to normal (p < 0.001,∗∗∗; p < 0.05,∗; and p < 0.001,∗∗∗). Panel (B) shows the significant downregulation of normalized IR/NIR MYH protein expression in XP-C1, XP-C2, and XP-C3 compared to normal (p < 0.01,∗∗; p < 0.01,∗∗ and p < 0.05,∗ respectively). Panel (C) shows the significant downregulation of normalized IR/NIR APE1 gene expression in XP-C2 fibroblast compared to normal (p < 0.0001,****). Statistical analysis was done by unpaired-t-test that allows the comparison between normal and each XP-C fibroblast (GraphPad Prism 8). IR, irradiated; NIR, Non-Irradiated.