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. 2020 Dec 10;10:21708. doi: 10.1038/s41598-020-78776-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human HSC isolated from normal human livers and timecourse of their in vitro activation. (A) Representative brightfield H&E stained images of four human donor livers used in the study, which depict normal tissue structure and architecture at X10 magnification. (B) Representative brightfield images of four human HSC preps used in the study showing quiescent HSC in top panel and fully activated HSC in the bottom panel. (C) Quantitative PCR carried out on time-course cDNA isolated from of human HSC preps used in the study showing αSMA transcript levels in (C) and Collagen1A1 in (D).