Figure 4.

Incorporation of FITC-labeled Pro-Hyp into fibroblasts. FITC-labeled Pro-Hyp was specifically incorporated into 75NTR-positive fibroblasts. Fibroblasts outside of the skin after 4 days of incubation were used. Cells were stained with DAPI in (A), antibody against p75NTR (red) in (B), fluorescence of FITC-labeled Pro-Hyp (green) in (C). Scale bar = 50 μm. High magnification picture of fibroblast incorporating FITC-labeled Pro-Hyp in (D). This figure was adapted with permission (Asai et al., 2020b).