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Potential G-quadruplexes exhibit heightened selective constraints in bat and pangolin related betacoronavirus. (A–C) Boxplot showing the difference of nucleotide RS scores in G-tract, other nucleotides, other guanines, and PG4 loops (**p ≤ 0.01, ****p ≤ 0.0001). (D) Test of the RS score difference between the fragments containing PG4s and the randomly selected fragments. The abscissa indicates the round of the test, while the ordinate represents the p-value for each round.
