TABLE 2.

Recent advances in single-cell multimodal sequencing technologies.

Method	Multi-omics	Literature	Application	Throughput
T-ATAC-seq	ATAC + RNA	Satpathy et al. (2018)	Immune profiling	Low
scCAT-seq	ATAC + RNA	Liu et al. (2019)	Embryonic development	Low
sci-CAR	ATAC + RNA	Cao et al. (2018)	NA	High
Paired-seq	ATAC + RNA	Zhu et al. (2019)	Brain development	High
scNMT-seq	Methylation + ATAC + RNA	Clark et al. (2018); Argelaguet et al. (2019)	Embryonic development	Low
scTrio-seq	Methylation + RNA + DNA copy number	Hou et al. (2016); Bian et al. (2018)	Tumor heterogeneity	Low
scCOOL-seq	Methylation + ATAC + DNA copy number	Guo et al. (2017); Li L. et al. (2018)	Embryonic development	Low
scNOMe-seq	Methylation + ATAC	Pott (2017)	Technology development	Low
Methyl-Hi C	Methylation + Hi C	Li G. et al. (2019)	NA	Low
CITE-seq	RNA + epitope	Stoeckius et al. (2017)	Immune profiling	High
Pi-ATAC	RNA + epitope	Chen et al. (2018)	Immune profiling	Low
REAP-seq	RNA + epitope	Peterson et al. (2017)	Immune profiling	High
ECCITE-seq	RNA + epitope + CRISPR	Mimitou et al. (2019)	Immune profiling	High
Slide-seq	Spatial + RNA	Rodriques et al. (2019)	Brain development	High
scDam&T-seq	Protein-DNA contacts + RNA	Rooijers et al. (2019)	NA	Low
Perturb-seq	RNA + CRISPR	Dixit et al. (2016)	Immune cell differentiation	High
Perturb-ATAC	ATAC + CRISPR	Rubin et al. (2019)	Keratinocyte differentiation	Low
ScarTrace	RNA + CRISPR	Alemany et al. (2018)	Lineage tracing	Medium
LINNAEUS	RNA + CRISPR	Spanjaard et al. (2018)	Lineage tracing	High
LARRY	RNA + exogenous barcode	Weinreb et al. (2020)	Lineage tracing	High
CellTagging	RNA + exogenous barcode	Biddy et al. (2018)	Lineage tracing	High
EMBLEM	ATAC + mtDNA	Xu et al. (2019)	Lineage tracing	Low
	ATAC + mtDNA	Ludwig et al. (2019)	Lineage tracing	Low