Table 1.
Primers’ design for RT-PCR.
| Names | Primer’s sequence | Distribution | AT(°C) | Productsize (bp) | Extensiontime (s) |
|---|---|---|---|---|---|
| Zeb1 | F: 5′-GCTTGTGATTTGTGTGACAAGA-3′ R: 5′-AATCGCATGTGTTCAATCAA- 3′ |
XM_017016603.1 6160-6305 |
60 | 146 | 34 |
| Vimentin | F: 5′-GGCGATGGCCCAGCTGTAAG-3′ R: 5′-CTGCTGTCCCGCCGATTGAG-3’ |
NM_003380.3 131-261 |
60 | 131 | 34 |
| CEA | F:5′-AGCCTCACTTCTAAACTTCT-3′R:5′-TCCCCTTTGTACCAGCTGTA-3′ | NM_001277163151-300 | 50 | 150 | 34 |
| GAPDH | F: 5′-CAATGACCCCTTCATTGACC-3′ R: 5′-TGGAAGATGGTGATGGGATT-3′ |
NM_ 002046.3 201-335 |
60 | 135 | 34 |
AT, annealing temperature.