FIGURE 7.

TFEB knockdown weakens the effects of liraglutide on lysosome biogenesis and lipid accumulation. (A) Western blot detection of TFEB and its downstream targets CTSB and LAMP1 in HepG2 cells expressing TFEB-siRNA or control-siRNA. Relative expression levels were normalized to β-actin levels. (B) Lipid droplets in HepG2 cells treated with TFEB-siRNA or control-siRNA were detected via labeling with the lipid dye BODIPY 493/503 (green). Nuclei were stained with DAPI (scale bars = 50 μm). (C) The TG content of HepG2 cells. The data are expressed as the mean ± SEM; n = 3. *P < 0.01, **P < 0.05 and ^#P < 0.01 vs. siCON + PA group; ^&P < 0.01 vs. siCON + PA + LG group.