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. 2020 Nov 27;11:503524. doi: 10.3389/fphar.2020.503524

FIGURE 8.

FIGURE 8

TFRD promotes the PDGF-BB-mediated osteogenic differentiation of BMSCs under stress conditions. (A) Representative images of ALP and ARS staining of the stretched or unstretched BMSCs at 7 and 14 days, respectively. (B), (C) Quantitative determination of ALP activity and the production of mineralized nodules. (D) Representative images and (E), (F) quantification of the protein levels of RUNX2 and OSX in the stretched or unstretched BMSCs treated with CM (TFRD) or CM (DMSO) for 7 days, following the exposure to 20 μg/ml function blocking anti-PDGF-BB antibody overnight. (G), (H) The mRNA expression levels of RUNX2 and OSX in the stretched or unstretched BMSCs treated with CM (TFRD) or CM (DMSO) in the presence or absence of function blocking anti-PDGF-BB antibody 7 days after osteogenic differentiation. The data are expressed as the mean ± SEM. All experiments were performed at least three times. *p < 0.05, **p < 0.01, ***p < 0.001, NS: not significant.