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. 2019 Sep 25;7(4):620–635. doi: 10.1016/j.gendis.2019.09.010

Figure 2.

Figure 2

ROS and LD comparison of HG versus NG conditions in both HCCs and CR-CSCs after 72 hrs from the beginning of the treatment. a) HCCs and CR-CSCs were collected and stained with CM-H2DCFDA, as reported in the Material and Methods section and analyzed by FACS. A higher increase in ROS was observed in HCCs compared to CR-CSCs. b) HCCs and CR-CSCs stained with LD540 were analyzed by FACS. Both samples showed a LD increase in HG, with HCCs being more affected. c) 3D reconstruction of both HCCs and CR-CSCs in NG and HG conditions stained with LD540 (green) for visualizing LDs and counterstained with NucBlue (blue) for imaging nuclei. In the inset Max Intensity Projection of the relative z-stack images used for the 3D reconstruction. All images were taken at the confocal microscopes, as reported in Materials and Methods (Section ROS and LD Quantification) and were mounted with Avizo 9.3. Scale bar is 10 μm. Note: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.