FIG 3.

TWIST1 expression is not repressed by E2 via methylation of the gene promoter. N/Tert-1 cells were treated with 1 μM decitabine (5-aza-cytidine) for 72 h. Afterward, cells were harvested and processed for RNA, which was reverse transcribed into cDNA. (A) qRT-PCR on N/Tert-1 cells for innate immune genes MX1 and IFIT1 illustrates robust restoration of expression following decitabine treatment. Results are expressed as fold change from that observed in the untreated vector control N/Tert-1 cells. (B) The same cDNA was then analyzed for TWIST1 expression. Unlike the innate immune genes, decitabine does not restore TWIST1 repression. Data in panels A and B represent the mean of two independent experiments, and error bars indicate standard error of the mean. *, P < 0.05.