FIG 5.

E2 expression correlates with attenuated ability to wound heal. (A) N/Tert-1 cells were plated and allowed to grow to near confluence. Afterward, a ∼1-mm scratch was made in the cell monolayer using a pipette tip. The wound gap was imaged and measured at the same field at 0, 12, and 20 h. Arrows have been added for clarity. Size bars have been added at the bottom right of each image to illustrate a length of 200 μm. (B) The remaining wound gap was calculated relative to time zero for each field. By 20 h, the vector and E6 and E7 cells have completely closed wound gaps, while the E2 and HPV16 cells retain considerable gaps. Results are expressed as fold change from that observed in the vector control N/Tert-1 cells (lane 1). Data represent the mean of at least three independent experiments, and error bars depict standard error of the mean. *, P < 0.05.