Skip to main content
. 2020 Dec 9;5(6):e00981-20. doi: 10.1128/mSphere.00981-20

FIG 6.

FIG 6

Expression of TWIST1 inversely correlates with E2 expression in head and neck cancer cell lines. (A) qRT-PCR for HPV16 E2 expression in UM-SCC-104 and UM-SCC-47 cell lines. Results are expressed as threshold cycle (2−ΔΔCT) using GAPDH as internal control. Integrated UM-SCC-47 exhibited no 2−ΔΔCT change over negative experimental control, illustrating no presence of E2 mRNA. (B) The same cDNA in panel A was analyzed for TWIST1 expression. Episomal UM-SCC-104 has lower TWIST1 transcription levels than UM-SCC-47. Results are expressed as relative fold change from that observed in UM-SCC-47 cells. (C) Western blot analysis was carried out on protein extracted from the cells in panels A and B. Decreased TWIST1 transcription leads to reduced expression on the protein level in UM-SCC-104 compared to UM-SCC-47 cells. GAPDH is shown as internal control. (D) Western blotting was repeated and quantitated, and TWIST1 protein expression was calculated relative to UM-SCC-47 protein levels using ImageJ. Data represent the averages of at least 3 independent experiments, and error bars indicate standard error of the mean. *, P < 0.05.