. Author manuscript; available in PMC: 2021 May 19.

Published in final edited form as: Biochemistry. 2020 May 7;59(19):1832–1844. doi: 10.1021/acs.biochem.0c00233

Table 5.

Kinetics of DHA, 14S-HpDHA, and 14S-HDHA with h15-LOX-1 and h12-LOX

h15-LOX-1 + substrate with	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (s⁻¹ μM⁻¹)	relative k_cat/K_M^a	fold difference^a
DHA	2.4 ± 0.6	6.7 ± 3	0.36 ± 0.08	1.0	1.0
14S-HpDHA	2.0 ± 0.04	18 ± 1	0.11 ± 0.006	0.31	−3.2
14S-HDHA	0.57 ± 0.01	18 ± 4	0.03 ± 0.002	0.080	−13
h12-LOX + substrate with	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (s⁻¹ μM⁻¹)	relative k_cat/K_M^a	fold difference^a
DHA	13 ± 0.2	0.90 ± 0.06	14 ± 0.8	1.0	1.0
14S-HpDHA	0.22 ± 0.02	88 ± 16	0.0024 ± 0.0002	0.00017	−5880
14S-HDHA	0.17 ± 0.03	210 ± 70	0.00080 ± 0.00003	0.00006	−16 700

Relative and fold difference of k_cat/K_M of enzyme with DHA set to 1.