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. 2020 Nov;8(22):1521. doi: 10.21037/atm-20-7144

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Validation of CA9 expression. (A) CA9 expression from the GEO dataset. (B) Expression of CA9 in cell lines at the mRNA level. (C) Expression of CA9 in cell lines at the protein level. (D) The level of CA9 protein expression in surgical tissues by immunohistochemistry. (E) Representative images of CA9 protein expression by immunohistochemistry (EnVision two-step method). ×200 magnification. Scale bars =100 µm. ***, P value <0.001. CA9, carbonic anhydrase 9; GEO, Gene Expression Omnibus (database); OTSCC, oral tongue squamous cell carcinoma.