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FAM83H-AS1 functioned as a sponge of miR-22-3p. (A) The putative binding sites between FAM83H-AS1 and miR-22-3p revealed through bioinformatics analysis; (B) RNA pull down assay validated the binding specificity of miR-22-3p to FAM83H-AS1; (C) qRT-PCR detection of miR-22-3p expression after different treatments. All experimental data are shown as mean ± SD from at least 3 independent assays. *, P<0.05 vs. control group; #, P<0.05 vs. AGEs group. qRT-PCR, quantitative real-time PCR.
