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. 2020 Nov;8(22):1518. doi: 10.21037/atm-20-7056

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Upregulation of miR-22-3p partially abolished the impact of FAM83H-AS1 on inflammatory pain. (A) The paw withdrawal thresholds of rats in different treatment groups; (B,C,D) Western blot was performed to measure the protein expression of IL-23 and IL-10 after different treatments; (E) the immunofluorescence assay was conducted to detect CD68 expression in macrophages; (F) the positive F4/80⁺CD11C⁺ macrophages were analyzed by flow cytometry. All experimental data are shown as mean ± SD from at least 3 independent assays. *, P<0.05 vs. IVDD group; ^#, P<0.05 vs. IVDD + FAM83H-AS1 group. qRT-PCR, quantitative real-time PCR; IVDD, intervertebral disc degeneration.