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. 2020 Dec 2;20(23):6890. doi: 10.3390/s20236890

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multiplexed diagnostics using quantum dot-linked immunosorbent assay (QLISA). Firstly, the surface of the quantum dots (QDs) is modified with specific antibodies using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) coupling, succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) conjugation, or streptavidin–biotin interaction. Clinical samples from patients are processed and loaded to a QLISA plate, which is coated with capture antibodies. Then, modified QDs are loaded and the plate is scanned using a microplate reader. Different QDs in the sample are excited with the same wavelength and several bands of photoluminescence are detected. The final step is the analysis of the acquired data.