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. 2020 Dec 4;21(23):9274. doi: 10.3390/ijms21239274

Figure 2.

Figure 2

PBCE, BDG10 and BDG30 polymer films, but not PBCE*, change the hBM-MSC shape. (a) Growth curves of cell proliferation on polymer films, TCP and GC. (b) XTT viability assay showed the absence of cytotoxicity in cells on polymer films, TCP and GC. (c) Percentage of live cells on polymer films, TCP and GC at each time point of culture. The results in a,b,c, are expressed as mean ± SD of three independent experiments, p < 0.05. (d) Representative fluorescence images of hBM-MSC nuclei (DAPI, blue) and F-actin (FITC-phalloidin, green) revealed the stem cell canonical fibroblast-like morphology on TCP and GC and the change of the cell shape only on PBCE-films. Scale bar 50 μm. (e) Representative fluorescence images of hBM-MSCs vinculin (red) and F-actin (FITC-phalloidin, green) showed the focal adhesion spots on CTR (TCP/GC) and polymer films. Scale bar 10 μm.