Figure 1.

Murine bone-marrow-derived mast cells (BMMCs) were susceptible to infection with wild-type recombinant vesicular stomatitis virus (VSV). BMMCs were left untreated in the cell culture medium or treated with a multiplicity of infection of 10 of wild-type VSV that carried a transgene encoding enhanced green fluorescent protein (GFP). (A) Ten hours later, BMMCs were harvested and stained for FCεRIα and c-Kit. GFP ⁺ cells, representative of VSV-infected BMMCs, were detected using flow cytometry. Dot plots are representative of four replicate experiments showing means and standard deviations of GFP⁺FCεRIα⁺c-Kit⁺ BMMCs. (B–F) Fluorescent microscopy (20×) of VSV-GFP-treated BMMCs (representative of three experimental replicates) at (B) 5 min, (C) 6 h, (D) 10 h, (E) 12 h, and (F) 24 h after exposure to VSV. Student’s t-tests were used to define statistical significance. **** p < 0.0001 for GFP⁺ cells in the VSV-GFP-infected versus uninfected cells.