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. 2020 Nov 27;21(23):9041. doi: 10.3390/ijms21239041

Figure 2.

Figure 2

Figure 2

Kinetics of cytokine production by murine bone-marrow-derived mast cells (BMMCs) after infection with a recombinant strain of vesicular stomatitis virus (rVSVΔm51). BMMCs were infected with rVSVΔm51 at a multiplicity of infection of 10. At 12, 16, and 20 h post-infection, the cells were stained for the surface markers FcεRIα and c-kit, as well as intracellular cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α. The cells were then analyzed using flow cytometry. Fluorescence minus one (FMA) controls were used to set gates to identify IL-6+ and TNF-α+ cells. Phorbol 12-myristate 13-acetate (PMA) and ionomycin were used as a positive control stimulus. Graphs and representative dot plots show means and standard deviations pooled from four experimental replicates of (A,B) IL-6+ and (C,D) TNF-α+ FcεRIα+ c-Kit+ mast cells at 12, 16, and 20 h post-infection. Two-way analysis of variance with Tukey’s multiple comparison test was used to define statistical significance as * p < 0.05; ** p < 0.001; **** p < 0.0001.