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. 2020 Nov 27;21(23):9041. doi: 10.3390/ijms21239041

Figure 3.

Figure 3

Cytokine responses of murine bone-marrow-derived mast cells (BMMCs) to recombinant vesicular stomatitis virus (rVSVΔm51) were dose-dependent. BMMCs were infected with rVSVΔm51 at a multiplicity of infection (MOI) of 0.01, 0.1, 1, 10, or 100. After 16 h, the cells were stained for the surface markers FcεRIα and c-kit as well as intracellular cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α. The cells were then analyzed by flow cytometry, and fluorescence minus one (FMO) controls were used to set gates to identify IL-6+ and TNF-α+ cells. Graphs and representative dot plots show means and standard deviations pooled from four experimental replicates of (A) IL-6 and (B) TNF-α expression in FcεRIα+c-Kit+ mast cells after infection with rVSVΔM51 or treatment with phorbol 12-myristate 13-acetate (PMA) and ionomycin as a positive control stimulus. Two-way analysis of variance with Tukey’s multiple comparison test was used to define statistical significance as ** p < 0.001; *** p < 0.0005; **** p < 0.0001.