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. 2020 Nov 27;21(23):9041. doi: 10.3390/ijms21239041

Figure 6.

Figure 6

Type I interferon receptor (IFNAR) signaling protected murine bone-marrow-derived mast cells (BMMCs) from recombinant vesicular stomatitis virus (rVSVΔm51)-induced cell death. IFNAR-intact, IFNAR-blocked, and IFNAR-knockout (IFNAR−/−) BMMCs were infected with rVSVΔm51 at a multiplicity of infection (MOI) of 10 or 100. Twelve, 16, and 20 h later, the cells were stained for the surface markers FcεRIα and c-kit. A cell viability dye was used to discriminate live and dead BMMCs by flow cytometry. Graphs show means and standard deviations pooled from four (IFNAR-intact and IFNAR-blocked BMMCs) or six (IFNAR−/− BMMCs) experimental replicates of the number of viable cells among FcεRIα+c-Kit+ mast cells at (A) 20 h post-infection with 10 or 100 MOI of the virus and (B) at 12, 16, and 20 h post-infection with 10 MOI of the virus. Two-way analysis of variance with Tukey’s multiple comparison test was used to define statistical significance as * p < 0.05; ** p < 0.001; *** p < 0.0005; **** p < 0.0001.