Figure 5.

Colocalization of GLUT4 and Rab13 in cross-section muscle fibers of rat soleus. In the cross-section muscle fibers of rat soleus treated with the therapy of olive leaf (TOL) in a dose-dependent manner, fibers are present that express Rab13. DAPI was used to stain nuclei (blue). Images (a(A–E)) show magnification 1000×. Scale bars indicate 20 μm. (a) Representative immunofluorescent pictures show the relationship between Rab13 and GLUT4 muscle fibers in Wistar rats: (a(A1–A4)) untreated; (a(B1–B4)) with SZT-induced diabetes; (a(C1–C4)) with SZT-induced diabetes and treated with TOL1, (a(D1–D4)) with TOL2, and (a(E1–E4)) with TOL3. (b) GLUT-Rab13 colocalization was assessed in the area of interest (0.014 mm²/4 μm slice × 3 slices/rat × 6 rats/group) by calculating the Pearson correlation coefficient using the JACoP plugin on ImageJ. Values are expressed as mean ± SD. One-way ANOVA followed by the post hoc Scheffé test: *** p < 0.001.