Figure 6.

TAN exhibited potent anti-inflammatory response and blockaded cell proliferation pathway. Measurement of different inflammatory markers on mice after PMA treatment estimated by ELISA analysis. (A) PGE₂, (B) IFN-γ, (C) TNF-α, (D) IL-6, (E) IL-1β, (F) Cox-2, (G) MCP-1, (H) MIP-2, (I) Keratinocyte chemoattractant (KC), (J) IL-4, (K) myeloperoxidase assay (MPO), Western blotting analysis on ear tissue homogenates showed that TAN was able to significantly reduce the inflammatory response challenged by PMA (10 μg/ear) and blockade the cell proliferation pathway validated. Densitometry analysis of these respective proteins were normalized by β-actin and evaluated through Image J software. The data are represented as mean ± S.D. of three independent experiments *** p < 0.001. PMA vs. control, TAN 10 mg/kg and 30 mg/kg vs. PMA. Statistical significance analysis was carried out through a one-way analysis of variance (ANOVA) prism.