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. 2020 Dec 10;10:21637. doi: 10.1038/s41598-020-78481-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative images from an uninjured and a transected nerve to show the orientation of neurofilaments at different zones. Scale bar, 500 µm (at upper right corner). Bar graph showing the quantification of misaligned fibers at proximal (b), injury (c) and distal (d) zones of transected nerves. Misaligned fibers are shown as the percentage of total number of fibers in each zone. n = 3/group, *P < 0.05, ***P < 0.001 vs. uninjured (CTL), ^#P < 0.05, ^###P < 0.001 vs. ST, ^$P < 0.05 vs. TG, and ^ƒƒƒP < 0.001 vs. SU. For other details, see Fig. 8.