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. 2020 Dec 10;10:21637. doi: 10.1038/s41598-020-78481-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative images from an uninjured and a transected nerve to show the blood vessels (left) and AngioTool reconstruction result (right) images. AngioTool images clearly depict the blood vessel architecture as red lines and their branching points as blue dots. Bar graph showing the quantification of blood vessels at proximal, injury, and distal zones of transected nerves. (b) Blood vessel density is shown as the percentage of number of blood vessel in total area. (c) Branching index of blood vessels is shown as the number of blood vessel junctions/mm². n = 3/group, **P < 0.01, ***P < 0.001 vs. uninjured (CTL), ^#P < 0.05, ^##P < 0.01 vs. ST, and ^$$P < 0.05 vs. TG. For other details, see Fig. 8.