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. Author manuscript; available in PMC: 2021 Oct 26.
Published in final edited form as: Dev Cell. 2020 Oct 9;55(2):224–236.e6. doi: 10.1016/j.devcel.2020.09.016

Figure 4. Hybrid cilium originates from parental centrioles.

Figure 4.

(A) Cartoon representation of centrinone A treatment in mouse tracheal multiciliated cells. (B) 2D projection micrograph of 3D-SIM volume of representative example of mouse tracheal multiciliated cells at ALI D20 treated with DMSO control or centrinone A labeled with anti-CNTRL (green) and anti-ZO-1 (red) antibodies. Arrowheads indicate CNTRL rings. Scale bar represents 5 μm. (C) Bar graphs representing percentage of cells with hybrid cilium in DMSO control (blue) and centrinone A-treated (pink) cells treated during basal cell expansion and throughout differentiation; n>800 over three independent biological replicates. Data are represented as mean ± SD. Statistical analysis was done using Cochran-Mantel-Haenszel test. **** p<0.0001. (D) Bar graph representing percentage of cells with none (red), one (yellow) or two (green) hybrid cilia in ALI D20 mouse tracheal multiciliated cells treated with DMSO control (left) or centrinone A (right); n>800 over three independent biological replicates. Data are represented as mean ± SD. Statistical analysis was done using Chi-square test. See also Figures S2 and S3.