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. 2020 Nov 30;21(23):9130. doi: 10.3390/ijms21239130

Figure 2.

Figure 2

(A) Long-term H2A.J accumulation following IR and ETO exposure: 53BP1. IFM micrographs show H2A.J and 53BP1 staining of NT and KD fibroblasts following 20 Gy (1 and 2 weeks post-IR) or ETO exposure (2 weeks post-ETO), compared to non-exposed controls. Quantification of H2A.J+ and 53BP1+ cells 1 and 2 weeks following IR or ETO exposure, compared to non-exposed controls. (B) Long-term H2A.J accumulation following IR and ETO exposure: SA-β-Gal. IHC micrograph show H2A.J and SA-β-Gal staining of NT and KD 24 h and 2 weeks following IR (20 Gy) or ETO exposure, compared to non-exposed controls. Quantification of H2A.J and SA-β-Gal cells 24 h, 1 week and 2 weeks following IR or ETO exposure compared to non-exposed controls. Data are presented as mean of three experiments ±SE. Significant statistical difference compared to non-irradiated controls (marked by asterisks alone) or between NT and KD cells (asterisks with square brackets): * p < 0.05; *** p < 0.001.