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. 2020 Dec 10;10:21634. doi: 10.1038/s41598-020-78502-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hybrid selection and identification. (a) Schema of hybrid generation. RST-DsRed + is co-cultured with Myo A8 CFP + or Myo D6-CFP + cell lines and spontaneous fusion DsRed+–CFP+ cells are isolated. Hybrid cells are selected following antibiotics treatment (Zeocin and Blasticidin). (b) Fluorescence panels of parental RST DsRed, Myo A8 CFP and Myo D6 CFP and all hybrids cells. Scale bar = 50 μm. (c) Phase contrast panel of parental RST DsRed, Myo A8 CFP and Myo D6 CFP and all hybrids cells. (Objective = 5x).