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. 2020 Dec 10;10:21634. doi: 10.1038/s41598-020-78502-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hybids are more aggressive and lead to lung metastases. (a) Tumour growth curve of RST/A8 H2, RST/D6 H1 and myoblast cell lines after subcutaneous xenograft in NSG mice. Myoblast cells did not lead to tumour growth in mice, as indicating by overlapping grey lines. (b) Subcutaneous primary tumours (black bars) and secondary tumours (grey bars) incidence. (c) Immunofluorescence of hybrids tumours and lung metastasis. Scale bars = 50 μm. (d) Tumour growth rate after injection of RST DsRed (n = 10) and hybrids (n = 6). Ns: non-significant. (e) HE staining of primary tumours and lung metastases developed from RST/A8 H2 and RST/D6 H1. (f) Immunohistochemistry staining of desmin, myogenin, transgelin and caldesmone on primary tumours of hybrids. Scale bar = 50 μm.