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. 2020 Dec 10;3:753. doi: 10.1038/s42003-020-01478-z

Fig. 4. Replacing GFP with TGP improved expression of two human membrane proteins in S. cerevisiae.

Fig. 4

Assessment of expression level was based on fluorescence counts (a, b), in-gel fluorescence (c, d), and relative FSEC intensities (e, f). Normalized FSEC traces are shown in g, h. Mean and standard deviation (a, b) or a representative (c–f) of three independent experiments on different colonies are shown. In e, g, f, and h, TGP traces are shown as black solid lines and scGFP traces are shown as red dashed lines. FSEC fluorescence-detection size exclusion chromatography, GFP green fluorescent protein, TGP thermostable GFP.