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. 2020 Dec 10;10:21641. doi: 10.1038/s41598-020-77806-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Diversity of microbiota composition in stool samples pre- and post- vitamin D supplementation. (A) Boxplots of Alpha-diversity indices: Observed OTUs; Chao1; Shannon and Inverse Simpson. Boxes represent the interquartile range (IQR) between the first and third quartiles (25th and 75th percentiles, respectively), and the horizontal line inside the box defines the median. Whiskers represent the lowest and highest values within 1.5 times the IQR from the first and third quartiles, respectively. Statistical significance was identified by the Wilcoxon test with false discovery rate (FDR)-Bonferroni corrected pairwise P values. *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001. (B) PCA on a weighted UniFrac dissimilarity matrix shows significant differences in β diversity of bacterial populations pre- and post- vitamin D supplementation, with higher variance post supplementation. *P < 0.05) The figure was generated using (RStudio v 1.2 with R v 3.6)⁸⁷.