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. 2020 Dec 10;10:21641. doi: 10.1038/s41598-020-77806-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Species level comparison within gut microbiota of responders and non-responders to vitamin D supplementation. (A) DESeq2 differential abundance analysis of significantly different OTUs post/pre in non-responders (p < 0.05, FDR-corrected); OTUs to the right of the zero line were more abundant in non-responders post- supplementation and OTUs to the left of the zero line were less abundant. (B–C) Comparison of relative abundance of B. acidifaciens in non-responders (B) and responders (C) pre- and post-vitamin D supplementation. Significant decrease in non-responders post supplementation (**P < 0.01). Responders show non-significant change. Statistical significance was identified by the Wilcoxon test with false discovery rate (FDR)-Bonferroni corrected pairwise P values. *P < 0.05; **P < 0.01). The figure was generated using (RStudio v 1.2 with R v 3.6)⁸⁷.