Figure 1.

Phylogeny reconstruction based on metallo-beta-lactamase (MBL) fold proteins. Phylogeny reconstruction was performed after amino acid sequence alignment with the Muscle program³⁴ with the Maximum-Likelihood method using FastTree³⁵, and tree was visualized with the MEGA 6 software³⁶. The amino acid sequences analyzed are Tupanvirus deep ocean protein AUL78925.1 and its homologs with the greatest BLASTp scores from the NCBI GenBank protein sequence database (nr) (see Supplementary Table 1), our sequence database of giant virus genomes, and previously described draft genome sequences from 14 Acanthamoeba species³⁷; a set of previously described MBL fold proteins²¹; and a set of sequences from the UniProtKB database¹, previously used for phylogeny reconstructions. Extended tree is available in Supplementary Fig. 1.