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Effect of BPA (0.5 μM for 48 h) on mRNA levels of eCB metabolic enzymes (NAPE-PLD, FAAH, DAGL-α, DAGL-β and MAGL) in exposed Sertoli cells, compared to vehicle-treated controls (Ctr). Gene expression was reported as 2−ΔΔCt values calculated by ΔΔCt method vs. Ctr, posed equal to 1.0. Expression levels of each gene were normalized to β-actin, and data were reported as means ± SEM of 5 independent experiments. ** p < 0.05 vs. Ctr.
