Table 1.
List of the primers used for qRT-PCR analysis.
| Gene | Corresponding Protein | PCR Primers | Annealing T (°C) |
Reference |
|---|---|---|---|---|
| Cnr1 | CB1 | Fw: 5′-CCAAGAAAAGATGACGGCAG-3′ Rev: 5′-AGGATGACACATAGCACCAG-3′ |
57 | [87] |
| Cnr2 | CB2 | Fw: 5′-TCGCTTACATCCTTCAGACAG-3′ Rev: 5′-TCTTCCCTCCCAACTCCTTC-3′ |
57 | [87] |
| Gpr55 | GPR55 | Fw: 5′-ATTCGATTCCGTGGATAAGC-3′ Rev: 5′- ATGCTGATGAAGTAGAGGC -3′ |
57 | [88] |
| Trpv1 | TRPV1 | Fw: 5′-TGAACTGGACTACCTGGAAC-3′ Rev: 5′-TCCTTGAAGACCTCAGCATC-3′ |
57 | [88] |
| Magl | MAGL | Fw: 5′-TTGTAGATACTGGAAGCCC-3′ Rev: 5′-ATGGTGTCCACGTGTTGCAGC-3′ |
62 | [52] |
| Faah | FAAH | Fw: 5′- AGATTGAGATGTATCGCCAG-3′ Rev: 5′- CTTCAGAATGTTGTCCCAC-3′ |
56 | [78] |
| Dagl-α | DAGL-α | Fw: 5′- AATGGCTATCATCTGGCTGAGC-3′ Rev: 5′-TTCCGAGGGTGACATTCTTAGC-3′ |
60 | [52] |
| Dagl-β | DAGL-β | Fw: 5′- TGTCAGCATGAGAGGAACCAT-3′ Rev: 5- CGCCAGGCGGATATAGAGC-3′ |
60 | Designed by Primer blast software |
| Nape-pld | NAPE-PLD | Fw: 5′-AAGTGTGTCTTCTAGGTTCTCC-3′ Rev: 5′-TTGTCAAGTTCCTCTTTGGAACC-3′ |
62 | [52] |
| Act-β | β-actin | Fw: 5′-TGTTACCAACTGGGACGA-3′ Rev: 5′-GTCTCAAACATGATCTGGGTC-3′ |
56 | [78] |