Figure 5.

Changes in biofilm formation of wild-type and mutant strains of Y. enterocolitica. Y. enterocolitica was cultured in LNNS (LBNS broth diluted 1:8 in distilled water) medium supplemented with 0.6 g/L L-arabinose at 26 °C. Biofilm formation was analyzed after 48 h of incubation by staining with crystal violet and measuring absorbance at 595 nm. Data are average values and standard deviations of triplicate experiments. The statistical comparison shows that there is a significant difference between WT and ΔwaaF; there is a significant difference between WT and ΔrcsF/ΔrcsC/ΔrcsB single mutant; there is a significant difference between ΔwaaF and ΔwaaF-ΔrcsF/ΔwaaF-ΔrcsC/ΔwaaF-ΔrcsB double mutant. An asterisk indicates a significant difference with ** p < 0.01.