Table 1.
Bacterial strains and plasmids used in this study.
| Strains and Plasmids | Relevant Characteristics | Sources |
|---|---|---|
| Y. enterocolitica | ||
| YE | WT, Serotype O:8, Biotype 1B, pYV- | [34] |
| YE-W | ΔwaaF | This study |
| YE-W+ | ΔwaaF, PBADwaaF; Ampr | This study |
| YE-F | ΔrcsF | [10] |
| YE-C | ΔrcsC | [10] |
| YE-B | ΔrcsB | [34] |
| YE-WF | ΔwaaF, ΔrcsF | This study |
| YE-WC | ΔwaaF, ΔrcsC | This study |
| YE-WB | ΔwaaF, ΔrcsB | This study |
| E. coli | ||
| S17-1 λpir | recA1, thi, pro, hsdR-M+, RP4:2-Tc::Mu-Kan::Tn7, λpir | [34] |
| DH5a | F-, φ80lacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | [34] |
| Plasmids | ||
| pDS132 | conditional replication vector; R6K origin, mobRK4 transfer origin, sucrose-inducible-sacB; Cmr | [34] |
| pDS132-ΔwaaF | upstream and downstream fragments of waaF gene were cloned into pDS132; Cmr | This study |
| pDS132-ΔrcsF | upstream and downstream fragments of rcsF gene were cloned into pDS132; Cmr | [10] |
| pDS132-ΔrcsC | upstream and downstream fragments of rcsC gene were cloned into pDS132; Cmr | [10] |
| pDS132-ΔrcsB | upstream and downstream fragments of rcsB gene were cloned into pDS132; Cmr | [34] |
| pBAD24 | AraC, promoter PBAD; Ampr | [34] |
| pBAD24-waaF | AraC, PBAD-waaF; Ampr | This study |
Amp, ampicillin; Cm, chloramphenicol; r, resistance.