Figure 1.

Two-week TRF does not significantly alter body weight, adipocyte size, or adipocyte size distribution in obese mice. (a) Body weights and food consumption (inset) were recorded on the indicated days. (b) Epididymal fat pads were excised and weighed post mortem. (c) Epidydimal adipose tissue sections were stained with hematoxylin and eosin (HE) to visualize tissue morphology. (d,e) Images were analyzed using AdipoCount to determine adipocyte number per image and individual adipocyte areas (adipocyte size). Mean adipocyte areas were calculated and adipocyte size frequencies were plotted. Scale bar represents 100 µm, n = 8–10.