Table 2.
Feeding Protocol | Ad Libitum | TRF |
---|---|---|
Lipid and lipoprotein metabolism | ||
Acox1 | 0.84 ± 0.06 | 0.77 ± 0.06 |
Cpt1a | 0.77 ± 0.06 | 0.79 ± 0.08 |
Ucp2 | 0.79 ± 0.06 | 0.69 ± 0.06 |
Cyp7a1 | 0.90 ± 0.13 | 1.00 ± 0.11 |
Bsep | 0.75 ± 0.08 | 0.61 ± 0.04 |
Fgf21 | 0.33 ± 0.08 | 0.23 ± 0.04 |
Hmgcr | 0.76 ± 0.08 | 0.81 ± 0.06 |
Ldlr | 0.90 ± 0.08 | 0.93 ± 0.08 |
Apoa1 | 0.73 ± 0.04 | 0.71 ± 0.04 |
ER homeostasis | ||
Grp78 | 1.20 ± 0.13 | 0.79 ± 0.12 * |
Eif2ak3 | 1.14 ± 0.08 | 1.04 ± 0.06 |
Atf6 | 0.76 ± 0.06 | 0.68 ± 0.06 |
Chop | 0.67 ± 0.04 | 0.59 ± 0.04 |
Xbp1s | 0.50 ± 0.06 | 0.48 ± 0.12 |
Txnip | 0.94 ± 0.13 | 0.84 ± 0.04 * |
Inflammation and fibrosis | ||
Cd68 | 1.09 ± 0.07 | 0.93 ± 0.11 |
Emr1 | 1.26 ± 0.07 | 1.22 ± 0.16 |
Mcp1 | 0.92 ± 0.17 | 0.62 ± 0.14 † |
Tnfa | 0.91 ± 0.12 | 0.86 ± 0.16 |
Fn1 | 0.55 ± 0.08 | 0.49 ± 0.06 |
1 Diet-induced obese male 129S6 mice were fed western diet ad libitum or on a time-restricted feeding (TRF) protocol for 14 days. Liver tissues were harvested and frozen at sacrifice (day 15). Gene expression was determined by RT-qPCR. Relative mRNA expression was normalized to Rplp0 and expression of each gene of interest was calculated using the standard curves method. Data are means ± SEM, * p < 0.05, † p = 0.21.