Table 2.
Liver tissue gene expression 1.
| Feeding Protocol | Ad Libitum | TRF |
|---|---|---|
| Lipid and lipoprotein metabolism | ||
| Acox1 | 0.84 ± 0.06 | 0.77 ± 0.06 |
| Cpt1a | 0.77 ± 0.06 | 0.79 ± 0.08 |
| Ucp2 | 0.79 ± 0.06 | 0.69 ± 0.06 |
| Cyp7a1 | 0.90 ± 0.13 | 1.00 ± 0.11 |
| Bsep | 0.75 ± 0.08 | 0.61 ± 0.04 |
| Fgf21 | 0.33 ± 0.08 | 0.23 ± 0.04 |
| Hmgcr | 0.76 ± 0.08 | 0.81 ± 0.06 |
| Ldlr | 0.90 ± 0.08 | 0.93 ± 0.08 |
| Apoa1 | 0.73 ± 0.04 | 0.71 ± 0.04 |
| ER homeostasis | ||
| Grp78 | 1.20 ± 0.13 | 0.79 ± 0.12 * |
| Eif2ak3 | 1.14 ± 0.08 | 1.04 ± 0.06 |
| Atf6 | 0.76 ± 0.06 | 0.68 ± 0.06 |
| Chop | 0.67 ± 0.04 | 0.59 ± 0.04 |
| Xbp1s | 0.50 ± 0.06 | 0.48 ± 0.12 |
| Txnip | 0.94 ± 0.13 | 0.84 ± 0.04 * |
| Inflammation and fibrosis | ||
| Cd68 | 1.09 ± 0.07 | 0.93 ± 0.11 |
| Emr1 | 1.26 ± 0.07 | 1.22 ± 0.16 |
| Mcp1 | 0.92 ± 0.17 | 0.62 ± 0.14 † |
| Tnfa | 0.91 ± 0.12 | 0.86 ± 0.16 |
| Fn1 | 0.55 ± 0.08 | 0.49 ± 0.06 |
1 Diet-induced obese male 129S6 mice were fed western diet ad libitum or on a time-restricted feeding (TRF) protocol for 14 days. Liver tissues were harvested and frozen at sacrifice (day 15). Gene expression was determined by RT-qPCR. Relative mRNA expression was normalized to Rplp0 and expression of each gene of interest was calculated using the standard curves method. Data are means ± SEM, * p < 0.05, † p = 0.21.