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. 2020 Dec 10;11:6324. doi: 10.1038/s41467-020-20031-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a RT-qPCR analysis for transcription across VLT and ORF63 loci in latently VZV-infected human iPSC-derived sensory neurons (HSN) cultures (n = 3). Data on individual HSN culture experiments are shown as unique symbols. b, c Latently VZV-infected HSN cultures were depleted of neurotrophic factors (NGF [nerve growth factor], GDNF [glial-derived neurotrophic factor], BDNF [brain derived neurotrophic factor] and NT-3 [neurotrophin-3]) and treated with anti-NGF antibody (Ab) for 14 days. In total, n = 40 independent cultures were subjected to reactivation stimuli. b Representative examples of a HSN cultures showing complete reactivation (2/40) and early/abortive reactivation (38/40) by infectious focus forming assay after transferring HSN onto ARPE-19 cells. c Representative examples of viral gene expression in cultures showing complete reactivation (n = 1 shown; white circle) and early/abortive reactivation (n = 5 shown; colored triangles). d, e HSN cultures were treated with d anisomycin (n = 6), or e DMSO as solvent control (n = 3) at both the somal and axonal compartment for 1 h, washed twice and cultured for 7 days before RT-qPCR analysis. Data on individual HSN culture experiments are shown as unique symbols and/or colors. Only VLT exon 1–2 is shown as representative of VLT. Source data are provided as a Source Data file (a–e).