Fig. 5. Effect of ectopic VLT-ORF63 expression on VZV gene expression in latently VZV-infected sensory neurons in vitro.

At 14 days after establishment of VZV latency in human iPSC-derived sensory neurons (HSN), following VZV genes were transduced by replication incompetent lentivirus vectors: empty vector (white), ORF63 (grey), VLT63-1 (purple) and VLT63-2 (cyan). Transduced HSN were cultured for 14 days (n = 4 replicates/vector) and subjected to RT-qPCR analysis. Technical duplicates were utilized per sample and all the biologically independent data is shown as unique symbols for a endogenous or transduced VZV genes, b IE genes, c E genes, and d L genes. Source data are provided as a Source Data file.