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. 2020 Nov 30;21(23):9096. doi: 10.3390/ijms21239096

Figure 1.

Figure 1

Irradiation (IR) increases intestinal permeability and leukocyte infiltration. (A) Mice were subjected to whole body IR (5 Gy). After 3 days, mice were injected with Evans blue solution (100 µL of 30 mg/mL) via the tail vein and sacrificed after 30 min. The intestines were collected and photographed. The amount of extracted Evans blue dye was quantified. Results were normalized by tissue weight (mean ± SD); n = 8 control and 8 irradiated mice, * p < 0.01. (B) Three days following IR, intestinal lamina propria cells were isolated and stained for myeloid cells (CD11b+), neutrophils (CD11b+Ly6G+), and macrophages (CD11b+F4/80+) and analyzed using flow cytometry. Graphs show the percent of the CD45+ cell population (mean ± SD); n = 5. * p < 0.01. (C) Representative results of the immunohistochemical staining of control and IR-treated intestines for myeloperoxidase (MPO) and F4/80 3 days after IR (7 Gy). Arrows, stained cells. Graphs show the MPO or F4/80 positive cell population (mean ± SD); n = 5. * p < 0.01.