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. 2020 Nov 30;21(23):9096. doi: 10.3390/ijms21239096

Figure 5.

Figure 5

Downregulation of EphA2 by dasatinib reduces IR-induced permeability and adhesion. (A) HUVECs and InEpCs were treated with dasatinib (500 nM) and then exposed to IR for 24 h. Expression levels of p-EphA2 (Ser897 and Tyr588), EphA2, p-FAK, ICAM1 and p-VE–cadherin were measured by Western blotting (left panel). (B) Twenty-four hours after IR (5 Gy), VE–cadherin expression and transmigration were analyzed in HUVECs with or without dasatinib (500 nM). HUVEC permeability is represented by the amount of FITC–dextran staining. * p < 0.05. (C) PKH26-labeled HUVECs (red) were exposed to IR (5 Gy) with or without dasatinib (500 nM) for 12 h, followed by co-culture with CFSE-labeled Jurkat cells (green). PKH26-labeled InEpCs (red) were exposed to IR with or without dasatinib for 12 h, followed by co-culture with CFSE-labeled THP1 cells (green). The adhesion rate was measured at 6 h. * p < 0.05.