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. 2020 Dec 5;21(23):9295. doi: 10.3390/ijms21239295

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A1 — Flow cytometric analysis of CD4Cre R26-tdT thymocytes. (A,B) Representative flow cytometric analyses of CD4Cre R26-tdT thymocytes stained with anti-CD4-FITC and anti-CD8-PerCP-Cy7 antibodies. (A) FSC/SSC plot for gating on thymocytes (left) and CD4/CD8 plot of the gated thymocytes for visualization of CD4/CD8 double-negative (DN), CD4 single-positive (CD4⁺ SP), CD8 single-positive (CD8⁺ SP) and CD4/CD8 double-positive (CD4⁺/CD8⁺ DP) T cells (right). (B) tdT expression analysis (left) of the gated thymocytes (see a left) and CD4/CD8 plots for individual CD4/CD8 expression analyses of tdT^neg (middle) and tdT⁺ thymocytes (right). tdT^neg thymocytes represent mainly DN and CD4⁺/CD8⁺ DP T cells (middle), whereas nearly all CD4⁺ SP and CD8⁺ SP thymocytes are tdT⁺ (right).