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Effect of COS on AMPK activation in MDCK cells. (a) Representative immunoblots of five separate experiments for the expression of p-AMPK, AMPK-α, and β-actin were performed. MDCK cells were incubated with COS at 10, 50, or 100 µg/mL or vehicle for 24 h before collecting samples for immunoblotting analysis. (b) Densitometry analysis of p-AMPK corrected by total AMPK and β-actin is depicted. Data are expressed as mean ± S.E.M. (n = 5). * p < 0.05; ** p < 0.01 compared with control.
