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. 2020 Dec 3;21(23):9240. doi: 10.3390/ijms21239240

Figure 5.

Figure 5

The role of HSP27 in W1CR cell resistance. Detection of protein levels of HSP27 (a) and p-HSP27 (b) in W1 and W1CR cells upon indicated treatment. (c) Knockdown of HSP27 in W1CR cells. Shown is a representative Western blot of HSP27 knockdown in W1CR cells, confirming the almost complete deletion of HSP27 in the knockdown cells compared to scrambled siRNA control. (d,e) The W1CR HSP27 knockdown cells display a similar sensitivity to cisplatin cytotoxicity as W1 cells, (d) shown in a representative MTT experiment and (e) statistically evaluated analyzing the EC50 values compared to the scrambled control cells and to W1CR wild type cells. (f) Use of chlorpromazine as an inhibitor of HSP27 confirms that W1 cells were not affected, while W1CR cells were sensitized independent of their COL1 effects. Data are means of at least n = 3 (±SEM), asterisks indicate statistical significance: * p < 0.05; ** p < 0.01; **** p < 0.0001.