Table 3.

Effect of GA and CA on the nitric oxide (NO) and arginase 1 (Arg1) activity in the supernatant of the spleen, ascites macrophages, and ascites fluid of mice bearing EAT.

Experimental Groupa	NO Levels in Supernatant of Macrophages or Ascites Fluid (SV ± SE)			Arg 1 Levels in Supernatant of Macrophages or Ascites Fluid (SV ± SE)
	Spleen Macrophages	Ascites Macrophages	Ascites Fluid	Spleen Macrophages	Ascites Macrophages	Ascites Fluid
Control	15.87 ± 0.91	10.16 ± 0.98	20.27 ± 0.77	10.64 ± 0.67	11.50 ± 0.95	161.28 ± 6.28
GA 40 mg/kg	12.39 ± 1.47	19.62 ± 0.86 *	10.57 ± 0.55 ***	7.89 ± 0.89 *	9.65 ± 0.86	85.83 ± 0.54 ***
GA 80 mg/kg	11.98 ± 0.94	23.71 ± 1.04 **	15.86 ± 0.94	8.56 ± 0.04 **	8.47 ± 0.77 *	114.57 ± 4.34 *
CA 40 mg/kg	10.69 ± 0.95 *	10.16 ± 1.12 ♦	14.71 ± 0.39 *	7.80 ± 1.11	8.38 ± 0.50 **	129.96 ± 2.49 ◊
CA 80 mg/kg	10.78 ± 1.04 *	12.02 ± 1.29	18.68 ± 0.66 ♦♦	6.54 ± 0.15 **	8.07 ± 0.07 ***♦	110.10 ± 4.80 *

^a Mice were injected intraperitoneally (ip) with 2.5 × 10⁶ viable EAT cells and treated with GA or CA at a dose of 40 mg/kg and 80 mg/kg ip in exponential tumor growth phase on day 5, 7, 9, 11. Mice were sacrificed on the 13th day and the supernatant of the spleen, ascitic macrophages, and ascites fluid were collected and the NO and Arg 1 levels were estimated by ELISA. The results are expressed as the mean value of each experimental group ± SE of the mean of three different observations; * p < 0.05,** p < 0.01, *** p < 0.001 as compared to the control group treated with saline; ^♦ p < 0.05, ^♦♦ p < 0.01 as compared to GA-40. Abbrevations: EAT, Ehrlich ascites tumor; GA, gallic acid; CA, caffeic acid.