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. 2020 Dec 7;21(23):9321. doi: 10.3390/ijms21239321

Figure 2.

Figure 2

Cell death induced by CC-115 treatment with/without IR. (A) Flow cytometry was used for apoptosis/necrosis analysis by V-APC/7-AAD staining. Unstained cells (Annexin-negative/7-AAD-negative) were defined as viable cells. Annexin-positive/7-AAD-negative cells were defined as apoptotic and Annexin-positive/7-AAD-positive cells as necrotic. Examples for gating at different CC-115 concentrations (0, 2 and 5 µmol/L) are shown, left: healthy-donor skin fibroblasts: SBLF9, right: melanoma cells: Mel624. (B) Graphs show proportion of dead cells (defined as sum of apoptotic and necrotic cells) at different CC-115 concentrations (0, 2 and 5 µmol/L) with/without IR (2 Gy) in skin fibroblast (SBLF9, SBLF7) and melanoma cells (RERO, ANST, A375M, LIWE, ICNI, PMelL, ARPA, HV18MK, Mel624). Each value represents mean ± SD (n = 3). Significance was determined by one-tailed Mann-Whitney U test * p ≤ 0.05.